FastQCFastQC Report
Sun 20 Nov 2022
CO_003-2-1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCO_003-2-1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150198
Sequences flagged as poor quality0
Sequence length25-345
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC59733.9767506890904003No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG58573.8995193011890974No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG48343.2184183544388074No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG46773.1138896656413535No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC45493.0286688238192254No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG42792.848906110600674No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG41252.7463747852834257No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA40882.721740635694217No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG28081.8695322174729356No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT27191.8102771008934875No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG25471.6957615946950026No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT25211.678451111199883No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA24501.631180175501671No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT23591.5705934832687518No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG23531.5665987563083394No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT22011.4653990066445626No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG21761.4487543109761782No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG21261.4154649196394093No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA19981.330244077817281No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT19921.326249350856869No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG19701.3116020186686905No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGC19611.3056099282280722No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT18601.2383653577277993No Hit
GCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT18471.2297101159802395No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC18021.1997496637771474No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG17831.1870996950691752No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT17691.17777866549488No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT17581.170454999400791No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG16841.1211867002223732No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT16821.1198551245689023No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC16711.1125314584748132No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC16471.0965525506331641No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT15281.0173237992516544No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGG15121.0066711940238884No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCA15051.002010679236741No Hit
CCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT14890.9913580740089748No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA14690.9780423174742673No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT14320.9534081678850583No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT14110.9394266235236155No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCG13750.915458261761142No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT13640.9081345956670529No Hit
CGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT13510.899479353919493No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT13120.8735136286768133No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC12830.8542057817014873No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT12180.810929572963688No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC11560.7696507277060947No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT11540.7683191520526239No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG10520.7004087937256156No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG9220.6138563762500167No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT8780.5845617118736601No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG8370.5572644109775097No Hit
CCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT8170.5439486544428022No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGC7320.48735668917029523No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA7280.4846935378633537No Hit
GCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT7260.483361962209883No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGG7070.47071199350191084No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG6990.4653856908880278No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6980.4647199030612924No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA6910.4600593882741448No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC6780.4514041465265849No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAG6740.4487409952196434No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCA6560.43675681433840663No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6440.4287673604175821No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAG6420.42743578476411137No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT6170.410791089095727No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCC6080.4047989986551086No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG5990.3988069082144902No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAG5580.3715096073183398No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT5500.36618330470445676No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAG5490.3655175168777214No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAG5360.3568622751301615No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4940.3288991864072757No Hit
CTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4060.27030985765456267No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGC3940.26232040373373816No Hit
GCCGCCTCCCTAATGTTACACACTGACTAG3490.2323599515306462No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAG3130.20839158976817268No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCC2280.15179962449566572No Hit
TCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2200.14647332188178272No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGA1670.1111865670648078No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAG1660.11052077923807242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)